Keywords
Juniperus phoenicea
Green plant
Medicinal plants
pigments and Chlorophyll
Abstract
As photosynthesis is the basic process during which light energy is absorbed and converted into organic matter, the importance of the plant pigment chlorophyll (a and b forms) as an intermediary in the transformation of the absorbed solar energy and its activity in the process of photosynthesis and synthesis of organic substances in plants is crucial. Therefore, this paper provides an overview of methods for monitoring the optical activity of chlorophyll molecules and methods (non-destructive and destructive) for quantification of chlorophyll in plants. These methods are used to estimate the effects of different stress factors (abiotic, biotic and xenobiotic) on the efficiency of photosynthesis and bioproductivity, aiming to assess the impact that these limiting factors have on the yield of various cultivars. Besides, those methods for the analysis of chlorophyll optical activity and/or content are appropriate for assessing the reaction of weed species to different agricultural practices (mineral nutrition, treatment by herbicides, etc.) and studies of different aspects of weed ecophysiology and their influence on crop harvest.
Drought stress is one of the most important factors affecting plant growth. Plant growth under drought stress may be enhanced by the application of microbial inoculation including plant growth-promoting Rhizobacteria. This research was conducted as a factorial experiment in a completely randomized design. The first factor included the bio-fertilizer (A) and control (without bio-fertilizer). The results showed that treatment microbial inoculation level had the highest effect on increasing photosynthetic pigments (p<0.01 ). While the lowest amount of photosynthetic pigments occurred in treatment A. The highest and lowest shoot fresh. weight was belonged to the treatments respectively (p<0.01). The results showed that the use of bio-fertilizers separately had more positive effects on the nutrients uptake of Juniperus phoenicea. In general, the results of this study suggest that growth-promoting bacteria as biofertilizers have a greater effect on growth, photosynthesis pigments and nutrient uptake of Juniperus phoenicea. The use of microbial inoculation indicated that the use of microbial isolates in agriculture increases plant productivity and its resistance to diseases and other influences such as drought, stress and lack of nutrients and provides it with important needs.
References
2- Ahmadi, U., D.A. Baker, 2000. Stomatal and nonstomatal factors limiting of photosynthesis in wheat under drought stress. Journal of Agriculture, 31; 813- 825.
3-Arancon, N., C.A. Edwardy., P. Bierman., C. Welch., J.D. Metzgar, 2004. Influences of vermicomposts on field strawberries: Effect on growth and yields. Bioresource Technology, 93; 145-153.
4- Arnon, D.I, 1949. Copper enzymes in isolated chloro plasts. Polyphenol oxidase in Beta volgaris. Plant Physiology, 1949; 24; 1-5.
5- Asghari, J., S.M. Ehteshami., S.M. Rajabi Darvishan., K. Khavazi, 2014. Study of root inoculation with plant growth promoting bacteria (PGPB) and spraying with their metabolites on chlorophyll content, nutrients uptake and yield in rice (Hashemi cultivar), Journal of Soil Biology, 2; 21-31.
6- Bashan, Y., H. Levanony, 1990. Current status of Azospirillum inoculation technology: Azospirillum as a challenge for agriculture. Candian Journal of Microbiology, 36; 591-608. Bray, R.H., L.T. Kurtz, 1954.
7- Determination of total, organic and available forms of phosphorus in soils. European Journal of Soil Science, 59; 39-45.
8- Bremner, J.M, 1996. Nitrogen total. In: Bartels, J.M amd J.M. Bigham (Ed.), Methods of Soil Analysis, Soil Science Society of America, Madison, WI; 1085- 1121.
9- Delshadi, S, 2015. Effects of plant growth promoting rhizobacteria on seed germination and growth of Bromus tomentellus, Onobrychis sativa and Avena sativa in drought stress. Range Management M.Sc. Thesis. University of Zabol.
10- Delshadi, S., M. Ebrahimi., E. Shirmohammadi, 2017. Influence of plant-growth-promoting bacteria on germination, growth and nutrients’ uptake of Onobrychis sativa L. under drought stress. Journal of Plant Interactions, 12; 200-208.
11- Ebrahimi, M., A. Ricki Maryshany., E. Shirmohammadi, 2016. Effect of extract of fast growing species Trifolium alexandrium L. on germination, photosynthetic pigments and nutrient uptake of Prosopis cineraria (L.) Druce. Ecopersia, 4; 1493- 1503.
12- Efeoglu, B., Y. Ekmekci., N.Cicek, 2009. Physiological responses of three maize cultivars to drought stress nd recouery. South African Journal of Botany, 75; 34-42.
13- Esitken, A., H.E. Yildiz., S. Ercisli., M. Donmez., M. Turan., A. Gunes, 2010. Effects of plant growth promoting bacteria (PGPB) on yield, growth and nutrient contents of organically grown strawberry. Scientia Horticulturae, 124; 62-66.
14- Fallah Nosrat Abad, A., S. Shariati, S, 2014. Effect of Pseudomonas and Bacillus bacteria on yield and nutrient uptake in comparison with chemical and organic fertilizers in wheat. Journal of Water and Soil, 28; 976-986.
15- Hamidi, A, 2000. Stomatal and nonstomatal limitations of photosynthesis under water stress conditions in wheat plant. Iranian Journal of Agriculture and Science, 31; 813-825.
16- Hayat, R., S. Ali., U. Amara., R. Khalid., I. Ahmed, 2010. Soil beneficial bacteria and their role in plant growth promotion: a review. Annals of Microbiology, 60; 579-598.
17- Khoshbakht, T., F. Bahadori., A. Khalighi., M. Moez Ardalan, 2011. Effect of plant growth promoting rhizobacteria on macro element content and yield of Aloe vera in green house condition. Journal of Plant Physiology, 2; 45-59.
18- Khosravi, H., H. Mahmoudi, 2013. Evaluation of effects of Azotobacter inoculation and manure on growth of rainfed wheat. Soil Management and Sustainable Production, 3; 205-219.
19- Kızılkaya, R, 2008. Yield response and nitrogen concentrations of spring wheat (Triticum aestivum) inoculated with Azotobacter chroococcum strains. Ecological Engineering, 33; 150-156.
20- Kloepper, J., D. Hume., F. Scher., C. Singleton., B. Tipping., E. Lalibert, 1987. Plant growth-promoting rhizobacteria on canola (rapeseed). Phytopathology, 71; 42-46.
21- Knudsen, D., G.A. Peterson., P. Pratt, 1982. Lithium, Sodium and Potassium. In: Page, A.L. (Ed.), Methods of Soil Analysis, American Society of Agronomy. Madison, 225-246.
22- Lo, I., D. Tsang., T. Yip., F. Wang., W.H. Zhang, 2011. Influence of injection conditions on EDDS-flushing of metal-contaminated soil. Journal of Hazardous Materials, 192; 667-675.
23- Madani, H., G. Naderi Brojerdi., H. Aghajani., A. Pazaki, 2011. Evaluating of chemical phosphate fertilizers and phosphor solublizing bacteria on seed yield, biological yield and tissues relative phosphorus content in winter rapseed (Brasssica napus L.). Journal of Agronomy and Plant Breeding, 6; 93-104.
24- Manivannan, P., C.A. Jaleel., C.X. Zhao., R. Somasundaram., M.M. Azooz., R. Panneerselvam, 2008. Variations in growth and pigment compositon of sunflower varieties under early season drought stress. Global Journal of Molecular Sciences, 3; 50- 56.
24- Martínez-Viveros, O., M.A. Jorquera., D.E. Crowley., G. Gajardo., M.L. Mora, 2010. Mechanisms and practical considerations involved in plant growth promotion by rhizobacteria. Journal of Soil Science Plant Nutrition, 10; 293-319.
25- Nadeem, S.M., M. Ahmad., Z. Zahir., A. Javaid., M. Ashraf, 2014. The role of mycorrhizae and plant growth promoting rhizobacteria (PGPR) in improving crop productivity under stressful environments. Biotechnology Advances, 32; 429-448.
26- Nazar, R., S. Umar., N. Khan., O. Sareer, 2015. Salicylic acid supplementation improves photosynthesis and growth in mustard through changes in proline accumulation and ethylene formation under drought stress. South African Journal of Botany, 98; 84-94.
27- Patten, C., B. Glick, 2002. Role of Pseudomonas putida indole acetic acid in development of the host plant root system. Applied and Environmental Microbiology, 68; 3795-3801.
28 - Rayan, J., G. Estefan., A. Rashid, 2001. Soil and plant analysis laboratory manual. International Center for Agricultural Research in the Dry Area (ICARDA), the National Agricultural Research Center. Aleppo, Syria.
29- Rhoades, J.D, 1996. Salinity: Electrical conductivity and total dissolved solids. In: Methods of soil analysis, American Society of Agronomy, Page, A.L, editor. Madison: WI; p. 417-435.
30 - Rodriguez, H., R. Fraga, 1999. Phosphate solubilizing bacteria and their role in plant growth promotion. Biotechnology Advances, 17; 319-339.
31 - Sadeghi, H., L. Rostami, 2016. Evaluating the physiological and hormonal responses of caper plant (Capparis spinosa) subjected to drought and salinity. Desert, 21; 49-55.
32 - Saharan, B.S., V. Nehra, 2011. Plant growth promoting rhizobacteria: a critical review. Life Science and Medical Research, 21; 1-30.
33 - Sandhya, V., S.K.Z. Ali., G. Reddy., B. Venkateswarlu, 2009. Alleviation of drought stress effects in sunflower seedlings by the exopolysaccharides producing Pseudomonas putida strain GAP-P45. Biology and Fertility of Soils, 46; 17-26.
34 - Thomas, G.W. 1996. Soil pH and soil acidity. In: Sparks, D.L. editor. Methods of soil analysis, part 3. Agron. Mongr. 9. 2nd ed. ASA and SSSA, Madison: WI; p. 475-490.
40 - Turan, M., M. Gulluce., G. Agar., F. Sahı̇n, 2012. Evaluation of PGPR strains on wheat yield and quality parameters. Power and Machinery. International Conference of Agricultural Engineering-CIGR-AgEng, agriculture and engineering for a healthier life, Valencia, Spain.
41 -Vacheron, J., G. Desbrosses., M. Bouffaud., B. Touraine., Y. Moenne-Loccoz., D. Muller., L. Legendre., F. Wisniewski-Dyé., C. Prigent-Combaret, 2013. Plant growth promoting rhizobacteria and root system functioning. Frontiers in Plant Science, 4; 1- 19.
42 - Wang, A., C. Luo., R. Yang., Y. Chen., Z. Shen., X. Li, 2012. Metal leaching along soil profiles after the EDDS application–A field study. Environmental Pollution, 164; 204-210.
43 - Yan, M, 2015. Seed priming stimulate germination and early seedling growth of Chinese cabbage under drought stress. South African Journal of Botany, 99; 88-92.
44 - Zahir, A., M. Arshad., W. Frankenberger, 2004. Plant growth promoting rhizobacteria: applications and perspectives in agriculture. Advances in Agronomy, 81; 97-168.
45 - Zandi Esfahan, E., H. Azarnivand, 2012. Effect of water stress on seed germination of Agropyron elongatum, Agropyron desertourm and Secale montanum. Desert, 17; 249-253.